验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30251-1-AP targets CRTAC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag32963 Product name: Recombinant human CRTAC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 198-332 aa of BC034245 Sequence: VVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDGKMVSRNVASGEMNSVLEILYPRDEDTLQDPAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cartilage acidic protein 1 |
| 别名 | ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320 |
| 计算分子量 | 661 aa, 71 kDa |
| 观测分子量 | 71-100 kDa |
| GenBank蛋白编号 | BC034245 |
| 基因名称 | CRTAC1 |
| Gene ID (NCBI) | 55118 |
| RRID | AB_3086281 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQ79 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRTAC1 antibody 30251-1-AP | Download protocol |
| IHC protocol for CRTAC1 antibody 30251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |