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Biotin-conjugated CRK Monoclonal antibody

CRK Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

ELISA

Conjugate

Biotin

CloneNo.

2B7E1

Cat No : Biotin-67035

Print datasheet

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

验证数据展示

  • ELISA experiment of Ag28793 using Biotin-67035

    ELISA experiment of Ag28793 using Biotin-67035

    Biotin-67035 tested by ELISA. Ag28793(CRK 41-304 aa)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-67035 (start dilution 1:500. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate,terminated with 2M H2SO4,then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive ELISA detected inAg28793

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67035 targets CRK in ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications ELISA
Tested Reactivity Human, mouse, rat
Immunogen CRK fusion protein Ag28793 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Synonyms Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46108
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.