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CRK Monoclonal antibody

CRK Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2B7E1

Cat No : 67035-1-Ig

Print datasheet

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

验证数据展示

  • WB analysis of HEK-293 using 67035-1-Ig

    WB analysis of HEK-293 using 67035-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67035-1-Ig targets CRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,ELISA
Tested Reactivity Human, mouse, rat
Immunogen CRK fusion protein Ag28793 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Synonyms Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
RRIDAB_2882350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

实验方案

Product Specific Protocols
WB protocol for CRK antibody 67035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols