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验证数据展示

  • WB analysis of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    WB analysis of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 82701-3-RR (CPT1B antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82701-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    IHC staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82701-3-RR (CPT1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82701-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CPT1B antibody included
  • IHC staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    IHC staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82701-3-RR (CPT1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82701-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CPT1B antibody included
  • IF Staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    IF Staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B antibody (82701-3-RR, Clone: 242881B6 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82701-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    IF Staining of mouse heart using 82701-3-RR (same clone as 82701-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B antibody (82701-3-RR, Clone: 242881B6 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82701-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82701-3-RR

    Affinity and Kinetic Characterization of 82701-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82701-3-RR against Human CPT1B were performed. The affinity constant is 5.70 nM.

CPT1B Recombinant monoclonal antibody, PBS Only

CPT1B Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 82701-3-PBS

产品说明书

CloneNo. 242881B6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein, CPT1 M

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CPT1B Ready-To-Use IHC Kit. CPT1B primary antibody included.

产品信息

82701-3-PBS targets CPT1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag17840

Product name: Recombinant human CPT1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-250 aa of BC131570

Sequence: FNTTRIPGKDTDVLQHLSDSRHVAVYHKGRFFKLWLYEGARLLKPQDLEMQFQRILDDPSPPQPGEEKLAALTAGGRVEWAQARQAFFSSGKNKAALEAIERAAFFVALDEESYSYDPEDEASLSLYGKALLHGNCYNRWF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carnitine palmitoyltransferase 1B (muscle)
别名 Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein, CPT1 M
计算分子量 567 aa, 64 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC131570
基因名称 CPT1B
Gene ID (NCBI) 1375
RRIDAB_3743415
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92523
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPT1B (carnitine palmitoyltransferase 1B) is an isoform of the mitochondrial enzyme carnitine palmitoyltransferase 1, primarily expressed in skeletal muscle and heart tissues, where it plays a critical role in the regulation of long-chain fatty acid oxidation. By catalyzing the conversion of long-chain acyl-CoA to acyl-carnitine, which is then transported into the mitochondrial matrix for β-oxidation, CPT1B serves as a rate-limiting enzyme in energy metabolism, particularly under conditions of high energy demand such as exercise. Its activity is allosterically inhibited by malonyl-CoA, allowing tight metabolic control, and genetic variations or dysregulation of CPT1B have been implicated in metabolic disorders, insulin resistance, and obesity, highlighting its importance in maintaining systemic energy homeostasis.

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