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验证数据展示

  • WB analysis of mouse heart using 82701-3-RR

    WB analysis of mouse heart using 82701-3-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 82701-3-RR (CPT1B antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 82701-3-RR

    IHC staining of mouse heart using 82701-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82701-3-RR (CPT1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1B antibody included
  • IHC staining of mouse heart using 82701-3-RR

    IHC staining of mouse heart using 82701-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82701-3-RR (CPT1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPT1B antibody included
  • IF Staining of mouse heart using 82701-3-RR

    IF Staining of mouse heart using 82701-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B antibody (82701-3-RR, Clone: 242881B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 82701-3-RR

    IF Staining of mouse heart using 82701-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B antibody (82701-3-RR, Clone: 242881B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CPT1B Recombinant antibody

CPT1B Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 82701-3-RR

产品说明书

CloneNo. 242881B6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein, CPT1 M

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy CPT1B Ready-To-Use IHC Kit. CPT1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82701-3-RR targets CPT1B in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 CPT1B fusion protein Ag17840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carnitine palmitoyltransferase 1B (muscle)
别名 Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein, CPT1 M
计算分子量 567 aa, 64 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC131570
基因名称 CPT1B
Gene ID (NCBI) 1375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92523
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPT1B antibody 82701-3-RRDownload protocol
IHC protocol for CPT1B antibody 82701-3-RRDownload protocol
IF protocol for CPT1B antibody 82701-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols