验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82701-3-RR targets CPT1B in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag17840 Product name: Recombinant human CPT1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-250 aa of BC131570 Sequence: FNTTRIPGKDTDVLQHLSDSRHVAVYHKGRFFKLWLYEGARLLKPQDLEMQFQRILDDPSPPQPGEEKLAALTAGGRVEWAQARQAFFSSGKNKAALEAIERAAFFVALDEESYSYDPEDEASLSLYGKALLHGNCYNRWF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | carnitine palmitoyltransferase 1B (muscle) |
| 别名 | Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein, CPT1 M |
| 计算分子量 | 567 aa, 64 kDa |
| 观测分子量 | 70-85 kDa |
| GenBank蛋白编号 | BC131570 |
| 基因名称 | CPT1B |
| Gene ID (NCBI) | 1375 |
| RRID | AB_3743415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92523 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPT1B (carnitine palmitoyltransferase 1B) is an isoform of the mitochondrial enzyme carnitine palmitoyltransferase 1, primarily expressed in skeletal muscle and heart tissues, where it plays a critical role in the regulation of long-chain fatty acid oxidation. By catalyzing the conversion of long-chain acyl-CoA to acyl-carnitine, which is then transported into the mitochondrial matrix for β-oxidation, CPT1B serves as a rate-limiting enzyme in energy metabolism, particularly under conditions of high energy demand such as exercise. Its activity is allosterically inhibited by malonyl-CoA, allowing tight metabolic control, and genetic variations or dysregulation of CPT1B have been implicated in metabolic disorders, insulin resistance, and obesity, highlighting its importance in maintaining systemic energy homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CPT1B antibody 82701-3-RR | Download protocol |
| IHC protocol for CPT1B antibody 82701-3-RR | Download protocol |
| WB protocol for CPT1B antibody 82701-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
