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验证数据展示

  • FC experiment of Jurkat using CL488-17760

    FC experiment of Jurkat using CL488-17760

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CORO1A (CL488-17760) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

CoraLite® Plus 488-conjugated CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-17760

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

CLABP, Clipin A, CLIPINA, Clipin-A, CORO1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman colon tissue, mouse spleen tissue
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17760-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17760 targets CORO1A in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12139

Product name: Recombinant human CORO1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-461 aa of BC110374

Sequence: MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFVALICEASGGGAFLVLPLGKTGRVDKNAPTVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLMLPLREPVVTLEGHTKRVGIVAWHTTAQNVLLSAGCDNVIMVWDVGTGAAMLTLGPEVHPDTIYSVDWSRDGGLICTSCRDKRVRIIEPRKGTVVAEKDRPHEGTRPVRAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERRCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDTGRRRAAPEASGTPSSDAVSRLEEEMRKLQATVQELQKRLDRLEETVQAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 1A
别名 CLABP, Clipin A, CLIPINA, Clipin-A, CORO1
计算分子量 461 aa, 51 kDa
观测分子量 57 kDa
GenBank蛋白编号BC110374
基因名称 CORO1A
Gene ID (NCBI) 11151
RRIDAB_3084008
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31146
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CORO1A antibody CL488-17760Download protocol
Standard Protocols
Click here to view our Standard Protocols
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