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CoraLite® Plus 488-conjugated CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17760

Print datasheet

Synonyms

CLABP, Clipin A, CLIPINA, CORO1, CORO1A, Coronin 1A, Coronin like protein A, Coronin like protein p57, FLJ41407, HCORO1, p57, TACO

验证数据展示

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-17760

    FC experiment of Jurkat using CL488-17760

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CORO1A (CL488-17760) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman colon tissue, mouse spleen tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17760-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17760 targets CORO1A in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse, rat
Immunogen CORO1A fusion protein Ag12139 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coronin, actin binding protein, 1A
Synonyms CLABP, Clipin A, CLIPINA, CORO1, CORO1A, Coronin 1A, Coronin like protein A, Coronin like protein p57, FLJ41407, HCORO1, p57, TACO
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31146
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CORO1A antibody CL488-17760Download protocol
FC protocol for CL Plus 488 CORO1A antibody CL488-17760Download protocol
Standard Protocols
Click here to view our Standard Protocols