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COMT Monoclonal antibody

COMT Monoclonal Antibody for FC, IF-P, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

FC, IF-P, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat No : 68181-1-Ig

Print datasheet

Synonyms

catechol O methyltransferase, COMT

验证数据展示

  • WB analysis using 68181-1-Ig

    WB analysis using 68181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HepG2 using 68181-1-Ig

    FC experiment of HepG2 using 68181-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human COMT (68181-1-Ig, Clone:1A1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse cerebellum using 68181-1-Ig

    IF Staining of mouse cerebellum using 68181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue
Positive IF detected inmouse cerebellum tissue
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68181-1-Ig targets COMT in WB, IF, FC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications FC, IF-P, WB, ELISA
Tested Reactivity Human, pig, rat, mouse
Immunogen COMT fusion protein Ag6502 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catechol-O-methyltransferase
Synonyms catechol O methyltransferase, COMT
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC000419
Gene Symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21964
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

实验方案

Product Specific Protocols
WB protocol for COMT antibody 68181-1-IgDownload protocol
IF protocol for COMT antibody 68181-1-IgDownload protocol
FC protocol for COMT antibody 68181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols