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验证数据展示

  • WB analysis using 68181-1-Ig

    WB analysis using 68181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of mouse cerebellum using 68181-1-Ig

    IF Staining of mouse cerebellum using 68181-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

COMT Monoclonal antibody

COMT Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68181-1-Ig

产品说明书

CloneNo. 1A1A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

1A1A4, catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68181-1-Ig targets COMT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6502

Product name: Recombinant human COMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-182 aa of BC000419

Sequence: MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGMKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catechol-O-methyltransferase
别名 1A1A4, catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6
计算分子量 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000419
基因名称 COMT
Gene ID (NCBI) 1312
RRIDAB_2935272
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21964
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

实验方案

Product Specific Protocols
FC protocol for COMT antibody 68181-1-IgDownload protocol
IF protocol for COMT antibody 68181-1-IgDownload protocol
WB protocol for COMT antibody 68181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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