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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

    WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

    IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

COMT Monoclonal antibody, PBS Only

COMT Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 68181-1-PBS

产品说明书

CloneNo. 1A1A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, Indirect ELISA

别名

1A1A4, catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68181-1-PBS targets COMT in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6502

Product name: Recombinant human COMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-182 aa of BC000419

Sequence: MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGMKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catechol-O-methyltransferase
别名 1A1A4, catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6
计算分子量 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000419
基因名称 COMT
Gene ID (NCBI) 1312
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21964
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

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