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验证数据展示

  • WB analysis of ROS1728 using 26984-1-AP (same clone as 26984-1-PBS)

    WB analysis of ROS1728 using 26984-1-AP (same clone as 26984-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26984-1-PBS in a different storage buffer formulation.

  • WB analysis using 26984-1-AP (same clone as 26984-1-PBS)

    WB analysis using 26984-1-AP (same clone as 26984-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26984-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 26984-1-AP (same clone as 26984-1-PBS)

    IF Staining of HeLa using 26984-1-AP (same clone as 26984-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Collagen Type X antibody (26984-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26984-1-PBS in a different storage buffer formulation.

Collagen Type X Polyclonal antibody, PBS Only

Collagen Type X Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 26984-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

COL10, COL10A1, Col10a 1, Collagen alpha-1(X) chain, Collagen type X alpha 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy COL10A1 Ready-To-Use IHC Kit. COL10A1 primary antibody included.

产品信息

26984-1-PBS targets Collagen Type X in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25741

Product name: Recombinant human COL10A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 518-585 aa of BC130621

Sequence: QAVMPEGFIKAGQRPSLSGTPLVSANQGVTGMPVSAFTVILSKAYPAIGTPIPFDKILYNRQQHYDPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type X, alpha 1
别名 COL10, COL10A1, Col10a 1, Collagen alpha-1(X) chain, Collagen type X alpha 1
计算分子量 680 aa, 66 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC130621
基因名称 COL10A1
Gene ID (NCBI) 1300
RRIDAB_3085918
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03692
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Collagen type X alpha 1 (COL10A1), a secreted, short-chain collagen, belongs to the collagen family, which is a major interstitial matrix component specifically expressed by hypertrophic chondrocytes. COL10A1 expression is elevated in many solid tumor types, such as colon cancer, esophagus cancer, and breast cancer, and displays vital roles in many critical cellular processes (PMID: 30154451, 25321476).

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