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验证数据展示

  • WB analysis of ROS1728 using 26984-1-AP

    WB analysis of ROS1728 using 26984-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 26984-1-AP

    WB analysis using 26984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26984-1-AP

    IHC staining of mouse liver using 26984-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26984-1-AP (COL10A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL10A1 antibody included

Collagen Type X Polyclonal antibody

Collagen Type X Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26984-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COL10, COL10A1, Col10a 1, Collagen alpha-1(X) chain, Collagen type X alpha 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, ROS1728 cells, HCT 116 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COL10A1 Ready-To-Use IHC Kit. COL10A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26984-1-AP targets Collagen Type X in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Collagen Type X fusion protein Ag25741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type X, alpha 1
别名 COL10, COL10A1, Col10a 1, Collagen alpha-1(X) chain, Collagen type X alpha 1
计算分子量 680 aa, 66 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC130621
基因名称 COL10A1
Gene ID (NCBI) 1300
RRIDAB_3085918
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen type X alpha 1 (COL10A1), a secreted, short-chain collagen, belongs to the collagen family, which is a major interstitial matrix component specifically expressed by hypertrophic chondrocytes. COL10A1 expression is elevated in many solid tumor types, such as colon cancer, esophagus cancer, and breast cancer, and displays vital roles in many critical cellular processes (PMID: 30154451, 25321476).

实验方案

Product Specific Protocols
WB protocol for Collagen Type X antibody 26984-1-APDownload protocol
IHC protocol for Collagen Type X antibody 26984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Orthop Surg Res

Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes

Authors - Longfei Li
View Article