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验证数据展示

  • FC experiment of HepG2 using CL594-66173

    FC experiment of HepG2 using CL594-66173

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CNPY2, MSAP (CL594-66173, Clone:1G8G11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CNPY2, MSAP Monoclonal antibody

CNPY2, MSAP Monoclonal Antibody for FC (Intra)
Cat No. CL594-66173

产品说明书

CloneNo. 1G8G11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66173-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66173 targets CNPY2, MSAP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6363

Product name: Recombinant human CNPY2,MSAP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-182 aa of BC065015

Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 canopy 2 homolog (zebrafish)
别名 canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC065015
基因名称 CNPY2
Gene ID (NCBI) 10330
RRIDAB_2919959
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2B0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

实验方案

Product Specific Protocols
FC protocol for CL594 CNPY2, MSAP antibody CL594-66173Download protocol
Standard Protocols
Click here to view our Standard Protocols