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CNPY2, MSAP Monoclonal antibody

CNPY2, MSAP Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8G11

Cat No : 66173-1-Ig

Print datasheet

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

验证数据展示

  • WB analysis using 66173-1-Ig

    WB analysis using 66173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HepG2 using 66173-1-Ig

    FC experiment of HepG2 using 66173-1-Ig

    1X10^6 HepG2 cells were stained with 0.20ug CNPY2, MSAP antibody (66173-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

  • IHC staining of human pancreas cancer using 66173-1-Ig

    IHC staining of human pancreas cancer using 66173-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66173-1-Ig

    IHC staining of human pancreas cancer using 66173-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66173-1-Ig

    IF Staining of HepG2 using 66173-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

  • WB analysis of HepG2 using 66173-1-Ig

    WB analysis of HepG2 using 66173-1-Ig

    WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, rat liver tissue, human placenta tissue, pig liver tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66173-1-Ig targets CNPY2, MSAP in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat, pig
Immunogen CNPY2, MSAP fusion protein Ag6363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name canopy 2 homolog (zebrafish)
Synonyms canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC065015
Gene Symbol CNPY2
Gene ID (NCBI) 10330
RRIDAB_2881568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2B0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

实验方案

Product Specific Protocols
WB protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IHC protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IF protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols