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验证数据展示

  • WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 13006-1-AP (same clone as 13006-1-PBS)

    IF Staining of A431 using 13006-1-AP (same clone as 13006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody (13006-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

CMAS Polyclonal antibody, PBS Only

CMAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13006-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, EC:2.7.7.43

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13006-1-PBS targets CMAS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3686

Product name: Recombinant human CMAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC016609

Sequence: MDSVEKGAATSVSNPRGRPSRGRPPKLQRNSRGGQGRGVEKPPHLAALILARGGSKGIPLKNIKHLAGVPLIGWVLRAALDSGAFQSVWVSTDHDEIENVAKQFGAQVHRRSSEVSKDSSTSLDAIIEFLNYHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytidine monophosphate N-acetylneuraminic acid synthetase
别名 CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, EC:2.7.7.43
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC016609
基因名称 CMAS
Gene ID (NCBI) 55907
RRIDAB_3085404
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFW8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

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