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CLOCK Recombinant antibody

CLOCK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82829-1-RR

产品说明书

CloneNo. 1I23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, HEK-293 cells, A431 cells, PC-3 cells, NIH/3T3 cells, rat testis tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82829-1-RR targets CLOCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLOCK fusion protein Ag30011 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 clock homolog (mouse)
别名 1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48
计算分子量 846 aa, 95 kDa
观测分子量110 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
RRIDAB_3086549
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

实验方案

Product Specific Protocols
WB protocol for CLOCK antibody 82829-1-RRDownload protocol
IHC protocol for CLOCK antibody 82829-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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