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验证数据展示

  • WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

    WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

    WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • Affinity and Kinetic Characterization of 82829-1-RR

    Affinity and Kinetic Characterization of 82829-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

CLOCK Recombinant antibody, PBS Only

CLOCK Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82829-1-PBS

产品说明书

CloneNo. 1I23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

产品信息

82829-1-PBS targets CLOCK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLOCK fusion protein Ag30011 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 clock homolog (mouse)
别名 1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48
计算分子量 846 aa, 95 kDa
观测分子量110 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15516
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).