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验证数据展示

  • WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

    WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

  • WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

    WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

    IP experiment of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

    IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

CLK1 Polyclonal antibody, PBS Only

CLK1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 20439-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

CDC-like kinase 1, CLK, EC:2.7.12.1, STY

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20439-1-PBS targets CLK1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14214

Product name: Recombinant human CLK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-143 aa of BC031549

Sequence: MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDC-like kinase 1
别名 CDC-like kinase 1, CLK, EC:2.7.12.1, STY
计算分子量 484 aa, 57 kDa
观测分子量 62 kDa
GenBank蛋白编号BC031549
基因名称 CLK1
Gene ID (NCBI) 1195
RRIDAB_2878686
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49759
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CLK1 (CDC-like kinase 1) is the dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. It phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. It can phosphorylate: SRSF1, SRSF3 and PTPN1 (PMID: 10480872; 19168442). It also regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PMID: 19168442). Elevated CLK1 activity is frequently detected in cancers, where it alters splice isoform balance to promote proliferation, invasion and chemoresistance, making it an emerging therapeutic target.

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