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验证数据展示

  • FC experiment of HEK-293 using CL647-26999

    FC experiment of HEK-293 using CL647-26999

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CITED1 (CL647-26999) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-26999

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

FC (Intra)

别名

CITED1, Melanocyte specific protein 1, MSG1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CITED1 Ready-To-Use IHC Kit. CITED1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26999-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-26999 targets CITED1 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat, mouse
免疫原

CatNo: Ag24643

Product name: Recombinant human CITED1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC004240

Sequence: MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMQLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
别名 CITED1, Melanocyte specific protein 1, MSG1
计算分子量 27 kDa
观测分子量 30 kDa
GenBank蛋白编号BC004240
基因名称 CITED1
Gene ID (NCBI) 4435
RRIDAB_2934965
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99966
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CITED1 antibody CL647-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols
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