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验证数据展示

  • FC experiment of HEK-293 using CL647-26999

    FC experiment of HEK-293 using CL647-26999

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CITED1 (CL647-26999) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-26999

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

FC (Intra)

别名

CITED1, Melanocyte specific protein 1, MSG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CITED1 Ready-To-Use IHC Kit. CITED1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26999-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-26999 targets CITED1 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat, mouse
免疫原 CITED1 fusion protein Ag24643 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
别名 CITED1, Melanocyte specific protein 1, MSG1
计算分子量 27 kDa
观测分子量 30 kDa
GenBank蛋白编号BC004240
基因名称 CITED1
Gene ID (NCBI) 4435
RRIDAB_2934965
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99966
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CITED1 antibody CL647-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols