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CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 26999-1-AP

Print datasheet

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

验证数据展示

  • WB analysis of rat testis using 26999-1-AP

    WB analysis of rat testis using 26999-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A375 using 26999-1-AP

    IP experiment of A375 using 26999-1-AP

    IP result of anti-CITED1 (IP:26999-1-AP, 4ug; Detection:26999-1-AP 1:300) with A375 cells lysate 3000ug.

  • IHC staining of human colon cancer using 26999-1-AP

    IHC staining of human colon cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CITED1 antibody included
  • IHC staining of human colon cancer using 26999-1-AP

    IHC staining of human colon cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CITED1 antibody included
  • IF Staining of HEK-293 using 26999-1-AP

    IF Staining of HEK-293 using 26999-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CITED1 antibody (26999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HEK-293T using 26999-1-AP

    FC experiment of HEK-293T using 26999-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human CITED1 (26999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inrat testis tissue, A375 cells, mouse testis tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman colon cancer tissue, human thyroid cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy CITED1 Ready-To-Use IHC Kit. CITED1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 26999-1-AP is CITED1 Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Immunogen CITED1 fusion protein Ag24643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Synonyms CITED1, Melanocyte specific protein 1, MSG1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
RRIDAB_2880718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99966
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. The CITED1 gene encodes a 27 kDa protein, but a 30 kDa band was also detected in the publication. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526, 15272279, 15843474).

实验方案

Product Specific Protocols
WB protocol for CITED1 antibody 26999-1-APDownload protocol
IHC protocol for CITED1 antibody 26999-1-APDownload protocol
IF protocol for CITED1 antibody 26999-1-APDownload protocol
IP protocol for CITED1 antibody 26999-1-APDownload protocol
FC protocol for CITED1 antibody 26999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation

Authors - Xin Gu
View Article