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CoraLite® Plus 488-conjugated CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7E3

Cat No : CL488-68030

Print datasheet

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1

验证数据展示

  • FC experiment of HeLa using CL488-68030

    FC experiment of HeLa using CL488-68030

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CISD1 (CL488-68030, Clone:1D7E3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68030-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68030 targets CISD1 in FC (Intra) applications and shows reactivity with Human, mouse, rat, chicken, rabbit samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat, chicken, rabbit
Immunogen CISD1 fusion protein Ag8560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CDGSH iron sulfur domain 1
Synonyms C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZ45
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CISD1 antibody CL488-68030Download protocol
Standard Protocols
Click here to view our Standard Protocols