CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat No : 68030-1-Ig

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Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1



经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, pig brain tissue, rabbit brain tissue, rat brian tissue, mouse brain tissue, chicken brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68030-1-Ig targets CISD1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, rabbit, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, chicken, rabbit, pig
Immunogen CISD1 fusion protein Ag8560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CDGSH iron sulfur domain 1
Synonyms C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
RRIDAB_2918772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZ45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

实验方案

Product Specific Protocols
WB protocol for CISD1 antibody 68030-1-IgDownload protocol
IHC protocol for CISD1 antibody 68030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols