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CoraLite® Plus 488-conjugated CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C12

Cat No : CL488-67901

Print datasheet

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

验证数据展示

  • FC experiment of Jurkat using CL488-67901

    FC experiment of Jurkat using CL488-67901

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIB1 (CL488-67901, Clone:1D2C12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CIB1 Ready-To-Use IHC Kit. CIB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67901-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67901 targets CIB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat, bovine samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat, bovine
Immunogen CIB1 fusion protein Ag31174 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name calcium and integrin binding 1 (calmyrin)
Synonyms Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99828
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CIB1 antibody CL488-67901Download protocol
Standard Protocols
Click here to view our Standard Protocols