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验证数据展示

  • FC experiment of Jurkat using CL488-67901

    FC experiment of Jurkat using CL488-67901

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIB1 (CL488-67901, Clone:1D2C12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of A549 using CL488-67901

    IF Staining of A549 using CL488-67901

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CIB1 antibody (CL488-67901, Clone: 1D2C12 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67901

产品说明书

CloneNo. 1D2C12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, bovine

应用

IF/ICC, FC (Intra)

别名

Calcium- and integrin-binding protein, Calcium and integrin-binding protein 1, Calmyrin, CIB, CIBP

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CIB1 Ready-To-Use IHC Kit. CIB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67901-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67901 targets CIB1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, bovine samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, bovine
免疫原

CatNo: Ag31174

Product name: Recombinant human CIB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-191 aa of BC000846

Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium and integrin binding 1 (calmyrin)
别名 Calcium- and integrin-binding protein, Calcium and integrin-binding protein 1, Calmyrin, CIB, CIBP
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000846
基因名称 CIB1
Gene ID (NCBI) 10519
RRIDAB_2923821
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99828
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CIB1 antibody CL488-67901Download protocol
Standard Protocols
Click here to view our Standard Protocols
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