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验证数据展示

  • WB analysis using 67901-1-Ig

    WB analysis using 67901-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67901-1-Ig

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CIB1 antibody included
  • IHC staining of human breast cancer using 67901-1-Ig

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CIB1 antibody included
  • FC experiment of Jurkat using 67901-1-Ig

    FC experiment of Jurkat using 67901-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CIB1 Monoclonal antibody (67901-1-Ig, Clone:1D2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67901-1-Ig

产品说明书

CloneNo. 1D2C12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, bovine

应用

WB, IHC, ELISA

别名

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Hela cells, K-562 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Human milk
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CIB1 Ready-To-Use IHC Kit. CIB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67901-1-Ig targets CIB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, bovine samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, bovine
免疫原 CIB1 fusion protein Ag31174 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium and integrin binding 1 (calmyrin)
别名 DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000846
基因名称 CIB1
Gene ID (NCBI) 10519
RRIDAB_2918657
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99828
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CIB1 antibody 67901-1-IgDownload protocol
IHC protocol for CIB1 antibody 67901-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols