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验证数据展示

  • IF Staining of A431 using CL488-16424

    IF Staining of A431 using CL488-16424

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHMP7 antibody (CL488-16424) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for IF/ICC
Cat No. CL488-16424

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Chromatin-modifying protein 7, Chromatin modifying protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 16424-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16424 targets CHMP7 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CHMP7 fusion protein Ag9625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHMP family, member 7
别名 Chromatin-modifying protein 7, Chromatin modifying protein 7
计算分子量 453 aa, 51 kDa
观测分子量 51-55 kDa
GenBank蛋白编号BC019110
基因名称 CHMP7
Gene ID (NCBI) 91782
RRIDAB_3672651
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUX9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP7 (Charged multivesicular body protein 7), it is expected to be located in the nucleus and cytoplasm, which is ubiquitinated in lymph node and appendix. It is reported that Lem2p (LEM2), a member of the LEM (Lap2- Emerin-Man1) family of inner nuclear membrane proteins, and the ESCRT-II/ESCRT- III hybrid protein Cmp7p (CHMP7), work together to recruit additional ESCRT-III proteins to holes in the nuclear membrane. CHMP7 protein is involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. It is a part of ESCRT III complex, which colocalizes with chromatin. The molecular weight of CHMP7 is 50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHMP7 antibody CL488-16424Download protocol
Standard Protocols
Click here to view our Standard Protocols