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KD/KO Validated

CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 16424-1-AP

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Synonyms

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

验证数据展示

WB analysis using 16424-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 16424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).
WB analysis using 16424-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of A431 using 16424-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A431 using 16424-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 16424-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A431 cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 2 publications below

产品信息

16424-1-AP targets CHMP7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	IF/ICC, IHC, WB, ELISA
Cited Applications	WB, IP, IF, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Immunogen	CHMP7 fusion protein Ag9625 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	CHMP family, member 7
Synonyms	CHMP family, member 7, CHMP7, Chromatin modifying protein 7
Calculated Molecular Weight	453 aa, 51 kDa
Observed Molecular Weight	51-55 kDa
GenBank Accession Number	BC019110
Gene Symbol	CHMP7
Gene ID (NCBI)	91782
RRID	AB_2079500
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WUX9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for CHMP7 antibody 16424-1-AP	Download protocol
IHC protocol for CHMP7 antibody 16424-1-AP	Download protocol
IF protocol for CHMP7 antibody 16424-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Nat Cell Biol Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation. Authors - Marina Vietri KD Validated View Article
human	WB	Dev Cell CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. Authors - Leandro N Ventimiglia KD Validated View Article
human	WB	Dev Cell The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Authors - Samuel S Wallis KD Validated View Article
human	WB	Curr Biol Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation. Authors - Yolanda Olmos KD Validated View Article
human	WB,IP	Elife CDK1 controls CHMP7-dependent nuclear envelope reformation. Authors - Alberto T Gatta KD Validated View Article
pig	WB,IF,IP	PLoS Pathog Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection. Authors - Chun-Chun Liu View Article

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CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

验证数据展示

WB analysis using 16424-1-AP

IHC staining of human kidney using 16424-1-AP

IHC staining of human kidney using 16424-1-AP

WB analysis using 16424-1-AP

IF Staining of A431 using 16424-1-AP

IF Staining of A431 using 16424-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.

CDK1 controls CHMP7-dependent nuclear envelope reformation.

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.