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验证数据展示

  • IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

    IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

    WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

CHCHD4 Monoclonal antibody, PBS Only

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66718-1-PBS

产品说明书

CloneNo. 3E4C6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CHCHD4, FLJ31709, MIA40

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66718-1-PBS targets CHCHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag15339

Product name: Recombinant human CHCHD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC033775

Sequence: MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 4
别名 CHCHD4, FLJ31709, MIA40
计算分子量 142 aa, 16 kDa
观测分子量 22 kDa
GenBank蛋白编号BC033775
基因名称 CHCHD4
Gene ID (NCBI) 131474
RRIDAB_2882069
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8N4Q1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

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