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CHCHD4 Monoclonal antibody

CHCHD4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3E4C6

Cat No : 66718-1-Ig

Print datasheet

Synonyms

CHCHD4, FLJ31709, MIA40

验证数据展示

  • WB analysis using 66718-1-Ig

    WB analysis using 66718-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66718-1-Ig

    IHC staining of human testis using 66718-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 66718-1-Ig

    IHC staining of human testis using 66718-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells, rat brain tissue, Ramos cells, Daudi cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66718-1-Ig targets CHCHD4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human, mouse, rat
Immunogen CHCHD4 fusion protein Ag15339 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Synonyms CHCHD4, FLJ31709, MIA40
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033775
Gene Symbol CHCHD4
Gene ID (NCBI) 131474
RRIDAB_2882069
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N4Q1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

实验方案

Product Specific Protocols
WB protocol for CHCHD4 antibody 66718-1-IgDownload protocol
IHC protocol for CHCHD4 antibody 66718-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols