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Conjugate
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验证数据展示

  • FC experiment of HeLa using CL488-66524

    FC experiment of HeLa using CL488-66524

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CGGBP1 (CL488-66524, Clone:2F5G9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CGGBP1 Monoclonal antibody

CGGBP1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66524

产品说明书

CloneNo. 2F5G9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, mouse

应用

FC (Intra)

别名

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CGGBP1 Ready-To-Use IHC Kit. CGGBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66524-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66524 targets CGGBP1 in FC (Intra) applications and shows reactivity with Human, Rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Rat, mouse
免疫原

CatNo: Ag1129

Product name: Recombinant human CGGBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-167 aa of BC005222

Sequence: MERFVVTAPPARNRSKTALYVTPLDRVTEFGGELHEDGGKLFCTSCNVVLNHVRKSAISDHLKSKTHTKRKAEFEEQNVRKKQRPLTASLQCNSTAQTEKVSVIQDFVKMCLEANIPLEKADHPAVRAFLSRHVKNGGSIPKSDQLRRTYLPDGYENENQLLNSQDC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CGG triplet repeat binding protein 1
别名 20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC005222
基因名称 CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2934474
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UFW8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CGGBP1 antibody CL488-66524Download protocol
Standard Protocols
Click here to view our Standard Protocols
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