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验证数据展示

  • WB analysis using 66524-1-Ig

    WB analysis using 66524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66524-1-Ig (CGGBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

CGGBP1 Monoclonal antibody

CGGBP1 Monoclonal Antibody for WB, ELISA
Cat No. 66524-1-Ig

产品说明书

CloneNo. 2F5G9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, mouse

应用

WB, ELISA

别名

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy CGGBP1 Ready-To-Use IHC Kit. CGGBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66524-1-Ig targets CGGBP1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, mouse
免疫原 CGGBP1 fusion protein Ag1129 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CGG triplet repeat binding protein 1
别名 20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC005222
基因名称 CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2881887
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UFW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

实验方案

Product Specific Protocols
WB protocol for CGGBP1 antibody 66524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols