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验证数据展示

  • IF Staining of hTERT-RPE1 using CL594-16268

    IF Staining of hTERT-RPE1 using CL594-16268

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 CEP63 antibody (CL594-16268) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CEP63 Polyclonal antibody

CEP63 Polyclonal Antibody for IF/ICC
Cat No. CL594-16268

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, Canine

应用

IF/ICC

别名

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CEP63 Ready-To-Use IHC Kit. CEP63 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16268-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, Canine
免疫原

CatNo: Ag9335

Product name: Recombinant human CEP63 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC014050

Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 63kDa
别名 centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386
计算分子量 703 aa, 81 kDa
观测分子量 57-75 kDa
GenBank蛋白编号BC014050
基因名称 CEP63
Gene ID (NCBI) 80254
RRIDAB_2919829
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MT8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

实验方案

Product Specific Protocols
IF protocol for CL594 CEP63 antibody CL594-16268Download protocol
Standard Protocols
Click here to view our Standard Protocols
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