验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16268-1-AP targets CEP63 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9335 Product name: Recombinant human CEP63 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC014050 Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 63kDa |
| 别名 | Centrosomal protein of 63 kDa, centrosomal protein 63kDa |
| 计算分子量 | 703 aa, 81 kDa |
| 观测分子量 | 57-75 kDa |
| GenBank蛋白编号 | BC014050 |
| 基因名称 | CEP63 |
| Gene ID (NCBI) | 80254 |
| RRID | AB_2077079 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MT8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP63 antibody 16268-1-AP | Download protocol |
| IP protocol for CEP63 antibody 16268-1-AP | Download protocol |
| WB protocol for CEP63 antibody 16268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Massive centriole production can occur in the absence of deuterosomes in multiciliated cells. | ||
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Dev Cell A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.
| ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. | ||
