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CEP128 Polyclonal antibody

CEP128 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24810-1-AP

Print datasheet

Synonyms

C14orf145, C14orf61, CEP128

验证数据展示

  • WB analysis of HEK-293T using 24810-1-AP

    WB analysis of HEK-293T using 24810-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 24810-1-AP (CEP128 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24810-1-AP

    IF Staining of HeLa using 24810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

  • IF Staining of HeLa using 24810-1-AP

    IF Staining of HeLa using 24810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

经过测试的应用

Positive WB detected inHEK-293T cells
Positive IF detected inHeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24810-1-AP targets CEP128 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen CEP128 fusion protein Ag20610 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 145
Synonyms C14orf145, C14orf61, CEP128
Calculated Molecular Weight 1094 aa, 128 kDa
Observed Molecular Weight128 kDa
GenBank Accession NumberBC045834
Gene Symbol C14orf145
Gene ID (NCBI) 145508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZU80
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Centrosomal protein of 128kDa is encoded by CEP128 gene in humans. CEP128 localizes to the subdistal appendages of the mother centriole and regulates TGF-β/BMP signaling at the primary cilium (PMID: 29514088). Centrosomal protein CEP128 plays a role in male fertility and its variants affected the ciliogenesis in testes (PMID: 35296684).

实验方案

Product Specific Protocols
WB protocol for CEP128 antibody 24810-1-APDownload protocol
IF protocol for CEP128 antibody 24810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols