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验证数据展示

  • WB analysis using 66996-1-Ig

    WB analysis using 66996-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 66996-1-Ig

    IF Staining of hTERT-RPE1 using 66996-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CEP63 Monoclonal antibody

CEP63 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66996-1-Ig

产品说明书

CloneNo. 2G12B2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

2G12B2, centrosomal protein 63kDa, Centrosomal protein of 63 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CEP63 Ready-To-Use IHC Kit. CEP63 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66996-1-Ig targets CEP63 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 CEP63 fusion protein Ag9227 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrosomal protein 63kDa
别名 2G12B2, centrosomal protein 63kDa, Centrosomal protein of 63 kDa
计算分子量 703 aa, 81 kDa
观测分子量 85 kDa
GenBank蛋白编号BC014050
基因名称 CEP63
Gene ID (NCBI) 80254
RRIDAB_2882313
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96MT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

实验方案

Product Specific Protocols
WB protocol for CEP63 antibody 66996-1-IgDownload protocol
IF protocol for CEP63 antibody 66996-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Neural Regen Res

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

Authors - Lin Yin
View Article