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验证数据展示

  • WB analysis using 66996-1-Ig (same clone as 66996-1-PBS)

    WB analysis using 66996-1-Ig (same clone as 66996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66996-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 66996-1-Ig (same clone as 66996-1-PBS)

    IF Staining of hTERT-RPE1 using 66996-1-Ig (same clone as 66996-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66996-1-PBS in a different storage buffer formulation.

CEP63 Monoclonal antibody, PBS Only

CEP63 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66996-1-PBS

产品说明书

CloneNo. 2G12B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

2G12B2, centrosomal protein 63kDa, Centrosomal protein of 63 kDa

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CEP63 Ready-To-Use IHC Kit. CEP63 primary antibody included.

产品信息

66996-1-PBS targets CEP63 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9227

Product name: Recombinant human CEP63 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC014050

Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrosomal protein 63kDa
别名 2G12B2, centrosomal protein 63kDa, Centrosomal protein of 63 kDa
计算分子量 703 aa, 81 kDa
观测分子量 85 kDa
GenBank蛋白编号BC014050
基因名称 CEP63
Gene ID (NCBI) 80254
RRIDAB_2882313
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96MT8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

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