CoraLite® Plus 488-conjugated CENPF Polyclonal antibody
CENPF Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | A549 cells, HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 28568-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-28568 targets CENPF in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC |
Tested Reactivity | Human |
Immunogen | CENPF fusion protein Ag29665 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | centromere protein F, 350/400ka (mitosin) |
Synonyms | AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779 |
Calculated Molecular Weight | 368 kDa |
Observed Molecular Weight | 350 kDa |
GenBank Accession Number | NM_016343 |
Gene Symbol | CENPF |
Gene ID (NCBI) | 1063 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P49454 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CENPF antibody CL488-28568 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |