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验证数据展示

  • WB analysis of HeLa using 28568-1-AP

    WB analysis of HeLa using 28568-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28568-1-AP

    IHC staining of human lung cancer using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28568-1-AP

    IHC staining of human lung cancer using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 28568-1-AP

    IHC staining of rat small intestine using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 28568-1-AP

    IHC staining of rat small intestine using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 28568-1-AP

    IHC staining of rat testis using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28568-1-AP

    IHC staining of mouse kidney using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28568-1-AP

    WB analysis using 28568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28568-1-AP

    IF Staining of HeLa using 28568-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of HeLa using 28568-1-AP

    IF Staining of HeLa using 28568-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

CENPF Polyclonal antibody

CENPF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28568-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

PRO1779, CENP-F, CENF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat small intestine tissue, human lung cancer tissue, mouse liver tissue, mouse kidney tissue, human pancreas cancer tissue, mouse small intestine tissue, rat skin tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28568-1-AP targets CENPF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag29665

Product name: Recombinant human CENPF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3011-3210 aa of NM_016343

Sequence: SVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centromere protein F, 350/400ka (mitosin)
别名 PRO1779, CENP-F, CENF
计算分子量 368 kDa
观测分子量350 kDa
GenBank蛋白编号NM_016343
基因名称 CENPF
Gene ID (NCBI) 1063
RRIDAB_2918176
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

实验方案

Product Specific Protocols
IF protocol for CENPF antibody 28568-1-APDownload protocol
IHC protocol for CENPF antibody 28568-1-APDownload protocol
WB protocol for CENPF antibody 28568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Transl Androl Urol

CENPF promotes the proliferation of renal cell carcinoma in vitro

Authors - Ji Zhang
  • KD Validated
View Article
humanWB

Nat Commun

CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes

Authors - Yanfen Zheng
View Article
humanIHC,IF

Oncol Lett

FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma

Authors - Peipei Li
View Article
humanWB

Cancer Cell Int

CENPF interaction with PLA2G4A promotes glioma growth by modulating mTORC1 and NF-κB pathways

Authors - Junhong Li
View Article
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