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验证数据展示

  • WB analysis using 84271-1-RR (same clone as 84271-1-PBS)

    WB analysis using 84271-1-RR (same clone as 84271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84271-1-RR (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

    WB analysis of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

    WB result of CDK1 antibody (84271-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1 transfected HeLa cells. This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

    IF Staining of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1 antibody (84271-1-RR, Clone: 240937C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84271-1-RR

    Affinity and Kinetic Characterization of 84271-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84271-1-RR against Human CDK1 were performed. The affinity constant is 34.3 nM.

  • Featured Product
  • KD/KO验证

CDK1 Recombinant monoclonal antibody, PBS Only

CDK1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84271-1-PBS

产品说明书

CloneNo. 240937C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CDC2, 240937C5, CDC28A, CDKN1, Cell division protein kinase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.

产品信息

84271-1-PBS targets CDK1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29018

Product name: Recombinant human CDK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-297 aa of BC014563

Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle 2, G1 to S and G2 to M
别名 CDC2, 240937C5, CDC28A, CDKN1, Cell division protein kinase 1
计算分子量 25 kDa
观测分子量 34 kDa
GenBank蛋白编号BC014563
基因名称 CDK1
Gene ID (NCBI) 983
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP06493
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.

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