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验证数据展示

  • WB analysis using 67575-1-Ig (same clone as 67575-1-PBS)

    WB analysis using 67575-1-Ig (same clone as 67575-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67575-1-Ig (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67575-1-Ig (same clone as 67575-1-PBS)

    FC experiment of HeLa using 67575-1-Ig (same clone as 67575-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK1 Monoclonal antibody (67575-1-Ig, Clone:2A11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

CDK1 Monoclonal antibody, PBS Only

CDK1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67575-1-PBS

产品说明书

CloneNo. 2A11E4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.

产品信息

67575-1-PBS targets CDK1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29018

Product name: Recombinant human CDK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-297 aa of BC014563

Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 2, G1 to S and G2 to M
别名 CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1
计算分子量 25 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC014563
基因名称 CDK1
Gene ID (NCBI) 983
RRIDAB_2882788
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06493
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

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