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验证数据展示

  • WB analysis using 86379-1-RR (same clone as 86379-1-PBS)

    WB analysis using 86379-1-RR (same clone as 86379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86379-1-RR (CDCA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86379-1-RR (same clone as 86379-1-PBS)

    IF Staining of HepG2 using 86379-1-RR (same clone as 86379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDCA2 antibody (86379-1-RR, Clone: 250980D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86379-1-RR

    Affinity and Kinetic Characterization of 86379-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86379-1-RR against Human CDCA2 were performed. The affinity constant is below 1 pM.

CDCA2 Recombinant monoclonal antibody, PBS Only

CDCA2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86379-1-PBS

产品说明书

CloneNo. 250980D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Cell division cycle-associated protein 2, Repo Man, Repo-Man

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86379-1-PBS targets CDCA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11967

Product name: Recombinant human CDCA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 512-863 aa of BC036214

Sequence: ATSDEDPNTNIMNINENKNIPKAKNKSESENEPKAGTDSPVSCASITEERVASDSPKPALTLQQGQEFSAGGQNAENLCQFFKISPDLNIKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSEDLGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSSSVGSSVEISLENSELFKDLSDAIEQTFQRRNSETKVRRSTRLQKDLENEGLVWISLPLPSTSQKAKRRTICTFDSSGFESMSPIKETVSSRQKPQMAPPVSDPENSQGPAAGSSDEPGKRRKSFCISTLANTKATSQFKGYRRRSSLNGKGESSLTALERIEHNGERKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle associated 2
别名 Cell division cycle-associated protein 2, Repo Man, Repo-Man
计算分子量113 kDa
观测分子量140 kDa
GenBank蛋白编号BC036214
基因名称 CDCA2
Gene ID (NCBI) 157313
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ69YH5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner.

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