CoraLite® Plus 488-conjugated CDC6 Monoclonal antibody

CDC6 Monoclonal Antibody for IF/ICC
Cat No. CL488-66021

产品说明书

CloneNo. 6F12D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

6F12D2, Cdc18 related protein, CDC18L, Cdc18-related protein, CDC6 related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected insodium arsenite treated HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66021-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66021 targets CDC6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CDC6 fusion protein Ag17958 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 6 homolog (S. cerevisiae)
别名 6F12D2, Cdc18 related protein, CDC18L, Cdc18-related protein, CDC6 related protein
计算分子量 560 aa, 63 kDa
观测分子量 65 kDa
GenBank蛋白编号BC025232
基因名称 CDC6
Gene ID (NCBI) 990
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99741
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDC6 antibody CL488-66021Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...