CDC6 Monoclonal antibody
CDC6 Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
6F12D2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, ROS1728 cells, HEK-293T cells, Jurkat cell, Raji cells |
| Positive IF detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66021-1-Ig targets CDC6 in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IF/ICC, WB, ELISA |
| Tested Reactivity | human, rat, mouse |
| Immunogen | CDC6 fusion protein Ag17958 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cell division cycle 6 homolog (S. cerevisiae) |
| Synonyms | Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6) |
| Calculated Molecular Weight | 560 aa, 63 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC025232 |
| Gene Symbol | CDC6 |
| Gene ID (NCBI) | 990 |
| RRID | AB_2881493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99741 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC6 antibody 66021-1-Ig | Download protocol |
| IF protocol for CDC6 antibody 66021-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
