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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CD9 Monoclonal antibody

CD9 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H7B9

Cat No : CL594-60232

Print datasheet

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

验证数据展示

  • IF Staining of human breast cancer using CL594-60232

    IF Staining of human breast cancer using CL594-60232

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL594-60232

    IF Staining of human breast cancer using CL594-60232

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL594-60232

    IF Staining of human breast cancer using CL594-60232

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated CD9 antibody (CL594-60232, Clone: 4H7B9 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60232-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-60232 is CD9 Fusion Protein expressed in E. coli.

Tested Applications IF-P
Tested Reactivity human, rat
Immunogen CD9 fusion protein Ag14529 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD9 molecule
Synonyms 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
RRIDAB_2919904
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21926
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

实验方案

Product Specific Protocols
IF protocol for CL594 CD9 antibody CL594-60232Download protocol
Standard Protocols
Click here to view our Standard Protocols