CoraLite® Plus 750 Anti-Mouse CD8a (53-6.7)
CD8a Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
53-6.7
验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65069-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-65069 targets CD8a in FC applications and shows reactivity with Mouse samples.
Tested Applications | FC |
Tested Reactivity | Mouse |
Immunogen | Spleen cells or thymocyte membranes 种属同源性预测 |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD8 antigen, alpha chain |
Synonyms | BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2 |
GenBank Accession Number | BC030679 |
Gene Symbol | Cd8a |
Gene ID (NCBI) | 12525 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P01731 |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 750 CD8a antibody CL750-65069 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |