CoraLite® Plus 488 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65069

产品说明书

CloneNo. 53-6.7

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse

应用

IF/ICC, FC and More (1)

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65069-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 4 publications below

产品信息

CL488-65069 targets CD8a in IF/ICC, FC applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用IF
经测试反应性 Mouse
免疫原 Spleen cells or thymocyte membranes 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8 antigen, alpha chain
别名 BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2
GenBank蛋白编号BC030679
基因名称 Cd8a
Gene ID (NCBI) 12525
RRIDAB_2883161
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP01731
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD8a antibody CL488-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
IF

Mol Cell Endocrinol

Melatonin enhances anti-tumor immunity by targeting macrophages PD-L1 via exosomes derived from gastric cancer cells

Authors - Kaifang Wang
IF

Front Chem

Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

Authors - Yinghui Shang
IF

J Control Release

Self-heating mitochondrion-induced free radical blast for immunogenic cell death stimulation and HCC immunotherapy

Authors - Mengyun Peng
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