• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for FC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2C6

Cat No : CL488-66866

Print datasheet

Synonyms

CD81, CD81 antigen, CD81 molecule, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

验证数据展示

  • IF Staining of human tonsillitis using CL488-66866

    IF Staining of human tonsillitis using CL488-66866

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-66866

    IF Staining of human tonsillitis using CL488-66866

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-66866

    FC experiment of Jurkat using CL488-66866

    1X10^6 Jurkat cells were stained with 0.2 ug CoraLite® Plus 488-conjugated Anti-Human CD81 (CL488-66866, Clone: 1G2C6) (green), or stained with 0.2 ug isotype control (black). Cells were fixed with 90% MeOH.

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66866-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66866 targets CD81 in IF, FC applications and shows reactivity with Human samples.

Tested Applications FC, IF-P
Tested Reactivity Human
Immunogen CD81 fusion protein Ag27298 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD81 molecule
Synonyms CD81, CD81 antigen, CD81 molecule, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_2919381
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60033
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD81 antibody CL488-66866Download protocol
Standard Protocols
Click here to view our Standard Protocols