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验证数据展示

  • FC experiment of human PBMCs using 98090-1-RR

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD72 Rabbit RecAb (98090-1-RR, Clone: 241300C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL750-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98090-1-RR

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit RecAb (98090-1-RR, Clone:241300C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98090-1-RR

    Affinity and Kinetic Characterization of 98090-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98090-1-RR against Human CD72 were performed. The affinity constant is 10.7 nM.

Anti-Human CD72 Rabbit Recombinant Antibody

CD72 Uni-rAb® Recombinant Antibody for FC
Cat No. 98090-1-RR

产品说明书

CloneNo. 241300C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

241300C1, B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98090-1-RR targets CD72 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD72 molecule
别名 241300C1, B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2
计算分子量 359 aa, 40 kDa
GenBank蛋白编号BC030227
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_3672237
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP21854
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
FC protocol for CD72 antibody 98090-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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