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验证数据展示

  • IF Staining of HepG2 using CL488-17224

    IF Staining of HepG2 using CL488-17224

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CD5L antibody (CL488-17224) at dilution of 1:100.

CoraLite® Plus 488-conjugated CD5L Polyclonal antibody

CD5L Polyclonal Antibody for IF/ICC
Cat No. CL488-17224

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

AIM, API6, CD5 antigen like, CD5 molecule like, CD5L, CT 2, IgM associated peptide, PRO229, SP ALPHA, Spalpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 17224-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17224 targets CD5L in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CD5L fusion protein Ag11062 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD5 molecule-like
别名 AIM, API6, CD5 antigen like, CD5 molecule like, CD5L, CT 2, IgM associated peptide, PRO229, SP ALPHA, Spalpha
计算分子量 347 aa, 38 kDa
观测分子量40 kDa
GenBank蛋白编号BC033586
基因名称 CD5L
Gene ID (NCBI) 922
RRIDAB_3083999
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDO43866
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD5L antibody CL488-17224Download protocol
Standard Protocols
Click here to view our Standard Protocols