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CoraLite® Plus 488-conjugated CD5L Polyclonal antibody

CD5L Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17224

Print datasheet

Synonyms

AIM, API6, CD5 antigen like, CD5 molecule like, CD5L, CT 2, IgM associated peptide, PRO229, SP ALPHA, Spalpha

验证数据展示

  • IF Staining of HepG2 using CL488-17224

    IF Staining of HepG2 using CL488-17224

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CD5L antibody (CL488-17224) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 17224-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17224 targets CD5L in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen CD5L fusion protein Ag11062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD5 molecule-like
Synonyms AIM, API6, CD5 antigen like, CD5 molecule like, CD5L, CT 2, IgM associated peptide, PRO229, SP ALPHA, Spalpha
Calculated Molecular Weight 347 aa, 38 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC033586
Gene Symbol CD5L
Gene ID (NCBI) 922
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDO43866
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD5L antibody CL488-17224Download protocol
Standard Protocols
Click here to view our Standard Protocols