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验证数据展示

  • WB analysis of LNCaP using 85950-1-RR (same clone as 85950-1-PBS)

    WB analysis of LNCaP using 85950-1-RR (same clone as 85950-1-PBS)

    WB result of CD58 antibody (85950-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD58 knockout LNCaP cells. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

  • WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

    WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 85950-1-RR (same clone as 85950-1-PBS)

    IHC staining of human tonsillitis using 85950-1-RR (same clone as 85950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85950-1-RR (CD58 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 85950-1-RR (same clone as 85950-1-PBS)

    IF Staining of K-562 using 85950-1-RR (same clone as 85950-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD58 antibody (85950-1-RR, Clone: 250250H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02208-1

    Cytometric bead array sample test of MP02208-1

    The mean CD58 concentration was determined to be 126.7 ng/mL in human serum.

  • Cytometric bead array standard curve of MP02208-1

    Cytometric bead array standard curve of MP02208-1

    Cytometric bead array standard curve of MP02208-1, CD58 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85950-1-PBS. Detection antibody: 85950-2-PBS. Standard: Eg4236. Range: 0.313-40 ng/mL.

  • Featured Product
  • KD/KO Validated

CD58 Recombinant monoclonal antibody, PBS Only (Capture)

CD58 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85950-1-PBS

产品说明书

CloneNo. 250250H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85950-1-PBS targets CD58 as part of a matched antibody pair:

MP02208-1: 85950-1-PBS capture and 85950-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg4236

Product name: Recombinant Human CD58 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-215 aa of BC005930

Sequence: FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD58 molecule
别名 Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3
计算分子量 28 kDa
观测分子量55-70 kDa
GenBank蛋白编号BC005930
基因名称 CD58
Gene ID (NCBI) 965
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19256
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

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