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验证数据展示

  • IF Staining of human tonsillitis using CL594-67278

    IF Staining of human tonsillitis using CL594-67278

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD5 antibody (CL594-67278, Clone: 4D12C10 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-67278

    IF Staining of human tonsillitis using CL594-67278

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD5 antibody (CL594-67278, Clone: 4D12C10 ) at dilution of 1:200.

CoraLite®594-conjugated CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IF-P
Cat No. CL594-67278

产品说明书

CloneNo. 4D12C10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF-P

别名

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67278-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67278 targets CD5 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原

CatNo: Ag11065

Product name: Recombinant human CD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-378 aa of BC027901

Sequence: DPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNPAGLAAG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD5 molecule
别名 CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
计算分子量 495 aa, 55 kDa
GenBank蛋白编号BC027901
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_2920105
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06127
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IF protocol for CL594 CD5 antibody CL594-67278Download protocol
Standard Protocols
Click here to view our Standard Protocols
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