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验证数据展示

  • IHC staining of human tonsillitis using 67278-1-Ig

    IHC staining of human tonsillitis using 67278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67278-1-Ig

    IHC staining of human tonsillitis using 67278-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67278-1-Ig

    IF Staining of human tonsillitis using 67278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67278-1-Ig

    IF Staining of human tonsillitis using 67278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 67278-1-Ig

产品说明书

CloneNo. 4D12C10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IHC, IF-P, ELISA

别名

4D12C10, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67278-1-Ig targets CD5 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11065

Product name: Recombinant human CD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-378 aa of BC027901

Sequence: DPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNPAGLAAG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD5 molecule
别名 4D12C10, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1
计算分子量 495 aa, 55 kDa
GenBank蛋白编号BC027901
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_2882546
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06127
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IF protocol for CD5 antibody 67278-1-IgDownload protocol
IHC protocol for CD5 antibody 67278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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