CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 67278-1-Ig

产品说明书

CloneNo. 4D12C10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IHC, IF-P, ELISA

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67278-1-Ig targets CD5 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD5 fusion protein Ag11065 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD5 molecule
别名 T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule
计算分子量 495 aa, 55 kDa
GenBank蛋白编号BC027901
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_2882546
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06127
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IHC protocol for CD5 antibody 67278-1-IgDownload protocol
IF protocol for CD5 antibody 67278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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