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验证数据展示

  • FC experiment of human PBMCs using 65613-1-MR

    FC experiment of human PBMCs using 65613-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO (UCHL1) Mouse IgG2a RecAb (65613-1-MR, Clone:UCHL1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG.Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RA (HI100) (CL647-65108, Clone: HI100).Cells were not fixed.

  • FC experiment of human PBMCs using 65613-1-MR

    FC experiment of human PBMCs using 65613-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO (UCHL1) Mouse IgG2a RecAb (65613-1-MR, Clone: UCHL1) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

  • IHC staining of human tonsillitis using 65613-1-MR

    IHC staining of human tonsillitis using 65613-1-MR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human tonsillitis using 65613-1-MR

    IHC staining of human tonsillitis using 65613-1-MR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included

Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Uni-rAbTM Recombinant Antibody for IHC, FC
Cat No. 65613-1-MR

产品说明书

CloneNo. UCHL1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, FC

别名

CD45, EC:3.1.3.48, GP180, L CA, LCA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65613-1-MR targets CD45RO in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原 IL-2 dependent T cell line, CA1 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 CD45, EC:3.1.3.48, GP180, L CA, LCA
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3670360
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

实验方案

Product Specific Protocols
IHC protocol for CD45RO antibody 65613-1-MRDownload protocol
FC protocol for CD45RO antibody 65613-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols